Development of astroglial cells in patterned neuronal cultures

Authors: Nam, Yoonkey¹; Brewer, Gregory J.²; Wheeler, Bruce C.³

Source: Journal of Biomaterials Science, Polymer Edition, Volume 18, Number 8, 2007 , pp. 1091-1100(10)

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Abstract:

The purpose of this work was to study the development of astroglial cells in patterned neuronal cultures. Hippocampal neurons, derived from embryonic stage (E18) rats and cultured in serum-free Neurobasal/B27 medium, grew to follow patterns of poly(D-lysine) created by micro-contact printing. The growth of the astroglial cells and the co-localization of neurons and astroglial cells were measured for up to one month using fluorescence immunostaining of neurons and astroglial cells. Neurons grew to form square patterns within 2 weeks, while astroglia only started to emerge in the same period. Astroglial cells continued to proliferate for a month following a general growth curve. Over 90% of the astroglial cell area co-localized with neurons (within 2 μm) at an early stage of astroglial development (13 DIV). Over the remaining period, astroglial cells proliferated and the co-localization was 80%. Hence, in these culture conditions astroglial cells develop 2-3 weeks later than neurons but remain highly co-localized with neurons.

Keywords: PATTERNED NEURONAL NETWORK IN VITRO; HIPPOCAMPAL NEURONS; ASTROGLIAL CELLS; SERUM-FREE CULTURE MEDIUM

Document Type: Research article

DOI: http://dx.doi.org/10.1163/156856207781494430

Affiliations: 1: Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology, Daejeon, South Korea; Nano Bioelectronics & Systems Research Center, Seoul, South Korea 2: Department of Medical Microbiology, Immunology and Cell Biology, Southern Illinois University School of Medicine, Springfield, IL, USA 3: Department of Bioengineering, Beckman Institute, University of Illinois at Urbana-Champaign, 405 N. Mathews Avenue, Urbana, IL 61801, USA

Publication date: 2007-08-01