Laser Microdissection and Pressure Catapulting (LMPC) in Paraffin Sections Mounted on Glass Slides. A Methodological Report
Authors: Nafe R.1; Gangnus R.2; Glienke W.3; Burgemeister R.2; Haar B.2; Pries A.2; Schlote W.4
Source: Pathology Research and Practice, Volume 199, Number 6, July 2003 , pp. 411-414(4)
Publisher: Urban & Fischer
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Abstract:
The technique of laser microdissection together with laser pressure catapulting (LMPC) is demonstrated in paraffin sections obtained from surgical specimens of brain tumors mounted on glass slides. A sufficient and precise application of microdissection techniques in tissue on glass slides is worthwhile, since it offers the possibility of a retrospective analysis of archived paraffin sections in histopathology. We could demonstrate a precise dissection of areas in tissues of different thicknesses (4
m and 20 m). Areas of tissue mounted directly on glass need to be dissected in a scanning mode in order to remove the total region in form of small tissue fragments row by row.This mode provided a precise microdissection of tissue areas of different sizes and shapes. A successful molecular biological analysis of the microdissected regions could be demonstrated. As an example for such an analysis, differential-PCR for detecting an amplification of the gene for the epidermal growth factor receptor (EGFR) was performed.
Document Type: Research article
DOI: 10.1078/0344-0338-00438
Affiliations: 1: Department Neuroradiology, Clinics of Goethe-University, Frankfurt am Main 2: P.A.L.M. Microlaser Technologies AG, Bernried, Germany 3: Department Hematology, Clinics of Goethe-University, Frankfurt am Main 4: Department Neuropathology (Edinger-Institute), Clinics of Goethe-University, Frankfurt am Main, Germany
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