IngentaConnect Immunohistochemical Expression of the Intracellular Component of ...

Authors: Caulet-Maugendre S.¹; Birolleau S.¹; Corbineau H.²; Bassen R.³; Desrues B.³; Bidon N.⁴; Delaval P.³; Ramée M-P.¹; Brichory F.⁴; Dazord L.⁴

Source: Pathology Research and Practice, Volume 197, Number 12, December 2001 , pp. 797-801(5)

Publisher: Urban & Fischer

Abstract:

Specified galectins are known to play a role in regulating cell proliferation, differentiation, adhesion and migration. Po66, a mouse IgG1 monoclonal antibody produced by immunization against squamous cell cancer, reacts against a carbohydrate-binding protein (Po66-CBP), recently shown to be a member of the galectin family with a strong homology with galectin-8 (PCTA-1), identified as a human tumor-associated antigen.

We studied Po66 in squamous metaplasia of the bronchi in order to determine whether it could be specifically involved in neoplastic conditions and if so, if it would be helpful in distinguishing metaplasia at risk of cancer.

Twenty-eight formalin-fixed, paraffin-embedded archival tissues of 17 metaplasias with SCC, 3 metaplasia with distant neoplastic disease and 8 metaplasias with an inflammatory process, were immunostained using a streptavidin biotin peroxydase method.

The squamous metaplasias were positively stained in non-neoplastic disease as well as in neoplastic processes. Expression was also observed in stromal and normal cells.

Po66-CBP was not associated with a pre-neoplastic character. We discussed the expression of this intra-cellular component of galectin-8 according to the functions of galectins in cellular differentiation, host reaction against tumor, and inflammation.

Keywords: Galectin; Squamous cell metaplasia; Dysplasia-in situ carcinoma; Lung neoplasm

Language: English

Document Type: Original article

DOI: 10.1078/0344-0338-00162

Affiliations: 1: Service d'Anatomie et de Cytologie pathologiques B, Centre Hospitalier-Régionalet Universitaire Pontchaillou, Rennes, France 2: Department of Cardio-Thoracic Surgery, Pontchaillou, Rennes, France 3: Department of Pneumology, Pontchaillou, Rennes, France 4: Centre Anti-cancéreux “Eugène Marquis”, Rennes, France

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Immunohistochemical Expression of the Intracellular Component of Galectin-8 in Squamous Cell Metaplasiaof the Bronchial Epitheliumin Neoplastic and Benign Processes