IL-24 is Expressed by Rat and Human Macrophages

Authors: Garn H.¹; Schmidt A.²; Grau V.³; Stumpf S.¹; Kaufmann A.¹; Becker M.¹; Gemsa D.¹; Siese A.¹

Source: Immunobiology, Volume 205, Number 3, July 2002 , pp. 321-334(14)

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Abstract:

Recently, a number of interleukin-10 (IL-10) homologues, among them IL-24 formerly known as melanocyte differentiation factor-7 (mda-7), has been described. Since IL-10 is released by macrophages and plays an important role in the resolution of inflammatory processes, we hypothesized that IL-24 might also be expressed in cells of the monocyte/macrophage lineage. We analyzed IL-24 expression on the mRNA and protein level in stimulated rat and human macrophages. In rat alveolar macrophages and NR8383 cells, IL-24 mRNA induction was observed following stimulation with LPS and IL-4 whereas TNF-a failed. Intracellular IL-24 protein was detected in unstimulated and IL-4 stimulated NR8383 cells. Also human blood monocytes showed a strong up-regulation of IL-24 mRNA following preparation which was enhanced by LPS and lowered by IL-10. Furthermore, infection of human monocytes with influenza A virus A/PR/8 caused an induction of IL-24 mRNA expression. In conclusion, our data show that IL-24 expression is induced in stimulated and infected rat and human macrophages, however, more insights into the functions of IL-24 are necessary to define its physiological relevance.

Language: English

Document Type: Original article

Affiliations: 1: Institute of Immunology 2: Institute of Pathology 3: Institute of Anatomy and Cell Biology, Philipps University, Marburg, Germany

Publication date: 2002-07-01