Delivery of protein antigens and DNA by attenuated intracellular bacteria

Authors: Gentschev I.^{1, 2, *}; Dietrich G.^{1, 3, 2}; Spreng S.^{4, 2}; Pilgrim S.²; Stritzker J.²; Kolb-Mäurer A.²; Goebel W.²

Source: International Journal of Medical Microbiology, Volume 291, Numbers 6-7, February 2002 , pp. 577-582(6)

Publisher: Urban & Fischer

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Abstract:

On the basis of attenuated intracellular bacteria, we have developed two delivery systems for either heterologous proteins or DNA vaccine vectors. The first system utilizes attenuated strains of Gram-negative bacteria which are engineered to secrete heterologous antigens via the agr -hemolysin secretion system (type I) of Escherichia coli.

The second system is based on attenuated suicide strains of Listeria monocytogenes, which are used for the direct delivery of eukaryotic antigen expression vectors into professional antigen-presenting cells (APC) like macrophages and dendritic cells in vitro and can be also used in animal models.

Keywords: Vaccine; Escherichia coli hemolysin secretion system; DNA vaccine delivery; attenuated bacteria

Language: English

Document Type: Research article

DOI: http://dx.doi.org/10.1078/1438-4221-00170

Affiliations: 1: These two authors contributed equally to the manuscript 2: Department of Microbiology, University of Wuerzburg, D-97074 Wuerzburg, Germany 3: Present address: Institute for Hygiene and Microbiology, University of Wuerzburg, D-97080 Wuerzburg, Germany 4: Present address: Max-Planck-Institut fuer Infektionsbiologie, Abteilung Molekulare Biologie, D-10117 Berlin, Germany *

Publication date: 2002-02-01