Protective effect of melatonin on β-cell damage in streptozotocin-induced diabetes in rats
The aim of the present study was the evaluation of possible protective effects of melatonin against β-cell damage in streptozotocin-induced diabetes in rats. Malondialdehyde levels and glutathione peroxidase activity were measured in pancreatic homogenates. Pancreatic β-cells were examined by immunohistochemical methods. Streptozotocin was injected intraperitoneally at a single dose of 60 mg/kg for induction of diabetes. Melatonin (200 μg/kg/day, ip) was injected for 3 days prior to administration of streptozotocin; these injections were continued until the end of the study (4 weeks). Streptozotocin induced a significant increase in malondialdehyde levels (p < 0.01) and a significant decrease in glutathione peroxidase activity (p < 0.05) in pancreatic tissue. Degeneration of islet cells and weak immunohistochemical staining of insulin was observed in diabetic rats. Treatment of diabetic rats with melatonin markedly reduced malondialdehyde production (p < 0.05) and increased glutathione peroxidase activity (p < 0.01) without affecting hyperglycemia. Increased staining of insulin and preservation of islet cells were apparent in the melatonin-treated diabetic rats. These data suggest that melatonin treatment has a therapeutic effect in diabetes by reduction of oxidative stress and preservation of pancreatic β-cell integrity.
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Document Type: Research Article
Department of Biochemistry, Abant Izzet Baysal University, Faculty of Medicine, Duzce, Turkey
Department of Histology and Embryology, Abant Izzet Baysal University, Faculty of Medicine, Duzce, Turkey
Department of Biochemistry, Gazi University, Faculty of Medicine, Ankara, Turkey
Department of Medical Biology, Abant Izzet Baysal University, Faculty of Medicine, Duzce, Turkey
Publication date: 2003-07-01