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Protective effect of melatonin on β-cell damage in streptozotocin-induced diabetes in rats

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The aim of the present study was the evaluation of possible protective effects of melatonin against β-cell damage in streptozotocin-induced diabetes in rats. Malondialdehyde levels and glutathione peroxidase activity were measured in pancreatic homogenates. Pancreatic β-cells were examined by immunohistochemical methods. Streptozotocin was injected intraperitoneally at a single dose of 60 mg/kg for induction of diabetes. Melatonin (200 μg/kg/day, ip) was injected for 3 days prior to administration of streptozotocin; these injections were continued until the end of the study (4 weeks). Streptozotocin induced a significant increase in malondialdehyde levels (p < 0.01) and a significant decrease in glutathione peroxidase activity (p < 0.05) in pancreatic tissue. Degeneration of islet cells and weak immunohistochemical staining of insulin was observed in diabetic rats. Treatment of diabetic rats with melatonin markedly reduced malondialdehyde production (p < 0.05) and increased glutathione peroxidase activity (p < 0.01) without affecting hyperglycemia. Increased staining of insulin and preservation of islet cells were apparent in the melatonin-treated diabetic rats. These data suggest that melatonin treatment has a therapeutic effect in diabetes by reduction of oxidative stress and preservation of pancreatic β-cell integrity.

Document Type: Research Article


Affiliations: 1: Department of Biochemistry, Abant Izzet Baysal University, Faculty of Medicine, Duzce, Turkey 2: Department of Histology and Embryology, Abant Izzet Baysal University, Faculty of Medicine, Duzce, Turkey 3: Department of Biochemistry, Gazi University, Faculty of Medicine, Ankara, Turkey 4: Department of Medical Biology, Abant Izzet Baysal University, Faculty of Medicine, Duzce, Turkey

Publication date: July 1, 2003


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