Skip to main content

Histochemical demonstration of the heterogeneity of the epithelium of proximal tubules in the chick mesonephros

Buy Article:

$28.45 plus tax (Refund Policy)

Abstract:



Proximal tubules (PT) in 7–10-day old chick mesonephros were examined histochemically to evaluate their structural and functional properties related to the absorption capacity of the epithelium and its possible alterations leading to cystically dilated tubules (CDT). Alkaline phosphatase activity at the apical cell membrane was demonstrated in varying intensities in large PT. A similar heterogeneity was also detected in the expression of proteoglycans (Lewisx antigen) localized in the apical part of the epithelial cell membrane but not in the basolateral membrane parts (β-catenin, Na+,K+-ATPase). In analogy, the ability to accumulate trypan blue was found in the same cell population. We hypothesize that epithelial cells in proximal tubules of nephrons with a defective apical cell membrane cause reduced fluid absorption and subsequent overfilling and dilation of the tubules.

Keywords: alkaline phosphatase; chick mesonephros; proximal tubule; trypan blue

Document Type: Original Article

DOI: https://doi.org/10.1078/0065-1281-00678

Affiliations: 1: Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic 2: Institute of Histology and Embryology, First Faculty of Medicine, Charles University, Prague, Czech Republic 3: State University of New York, Cobleskill NY 12043, USA

Publication date: 2002-12-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more