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Comparison of the TUNEL, lamin B and annexin V methods for the detection of apoptosis by flow cytometry

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Abstract:



The present study was performed in order to compare the sensitivity of 3 apoptosis-detection methods using a model cell culture after induction of apoptosis. Evaluation was performed by means of flow cytometry and light microscopy. It appeared that both TUNEL and annexin V methods are sensitive and specific and produced similar data in all measurements. The immunocytochemical detection of lamin B was less reliable than the other methods.

Keywords: TUNEL; annexin V; apoptosis; flow cytometry; lamin B

Document Type: Original Article

DOI: https://doi.org/10.1078/0065-1281-00674

Affiliations: Department of Histology and Embryology, Olomouc, Czech Republic

Publication date: 2002-12-01

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