Short coherence digital holography for 3D microscopy
An optical system based on short coherence digital holography suitable for 3D microscopic investigations is described. An interferometer is built by using a short coherence laser (coherence length 50 micrometers). The sample is located in one arm of the interferometer, and the other arm is used as reference. The interference occurs only when the path lengths of the reference and measurement arms are matched within the coherence length of the laser. This interference pattern (hologram) is recorded on a CCD faceplate. The reference mirror position is shifted and, a sequence of holograms is recorded. These are then reconstructed numerically by using the wave propagation relation. The reconstruction contains the 3D information of the object. Experimental results showing the difference between a white light image and a short coherence hologram are presented.
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