Immunohistochemical study on inducible type of nitric oxide (iNOS), basic fibroblast growth factor (bFGF) and tumor growth factor-1 (TGF-1) in arteritis induced in rats by fenoldopam and theophylline, vasodilators

Authors: Ikegami H.^{1, 2}; Kajikawa S.¹; Ito K.¹; Nii A.¹; Okamiya H.¹; Nakayama H.²; Doi K.¹

Source: Experimental and Toxicologic Pathology, Volume 54, Number 1, July 2002 , pp. 1-7(7)

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Abstract:

Arteritis induced in rats by vasodilators, fenoldopam and theophylline, was examined immunohistochemically for expressions of inducible type of nitric oxide synthase (iNOS), basic fibroblast growth factor (bFGF) and tumor growth factor- beta 1 (TGF- beta 1). Rats were administered fenoldopam for 24 hours by intravenous infusion with or without following repeated daily oral administrations of theophylline. Irrespective of theophylline administration, iNOS antigens were remarkably abundant in ED-1-positive cells on day 5 and 8 post-fenoldopam-infusion (DPI); bFGF antigens were remarkably abundant in ED-1- positive cells on 1 and 3 DPI; TGF- beta 1 antigens were observed in ED-1-positive cells on and after 5 DPI. These results suggest that the peak expression of iNOS antigen was followed by that of bFGF antigen, and bFGF may have a suppressive effect on iNOS expression in these rat arteritis models. On the other hand, TGF- beta 1 was not considered to have a suppressive effect on iNOS expression in these models.

Keywords: Arteritis; rat; immunohistochemistry; inducible type of nitric oxide (iNOS); basic fibroblast growth factor (bFGF); tumor growth factor- beta 1 (TGF- beta 1); fenoldopam; theophylline; vasodilator

Language: English

Document Type: Original article

DOI: http://dx.doi.org/10.1078/0940-2993-00238

Affiliations: 1: Safety Research Laboratories, Yamanouchi Pharmaceutical Co., Ltd., Tokyo, Japan 2: Department of Veterinary Pathology, Faculty of Agriculture, The University of Tokyo, Japan

Publication date: 2002-07-01