Concanavalin A and the motile behaviour of the ciliate Tetrahymena

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Abstract:

Concanavalin A (Con A) is a plant lectin that inhibits the persistent swimming behaviour of Tetrahymena cells by increasing ciliary reversals. Con A also increases ciliary reversals following chemosensory adaptation when it acts as a chemoattractant. The Con A effect is inhibited by mannose suggesting that Con A binds to glycoproteins in cellular membranes. In order to isolate these glycoproteins membrane proteins were fractionated by Con A-Sepharose affinity chromatography. A ciliary membrane protein denoted p66 was purified by two-step affinity chromatography from a whole cell Triton X-100 extract of Tetrahymena thermophila.

Document Type: Research Article

DOI: http://dx.doi.org/10.1078/0932-4739-00009

Affiliations: 1: Institute of Medical Biochemistry and Genetics, Biochemistry Laboratory, Department B, University of Copenhagen, The Panum Institute, Blegdamsvej 3C, DK-2200 Copenhagen N, Denmark;, Email: leick@imbg.ku.dk 2: Institute of Medical Biochemistry and Genetics, Biochemistry Laboratory, Department B, University of Copenhagen, The Panum Institute, Blegdamsvej 3C, DK-2200 Copenhagen N, Denmark;, Email: leick@imbg.ku.dk 3: The Protein Laboratory, University of Copenhagen, The Panum Institute, Blegdamsvej 3C, DK-2200 Copenhagen N, Denmark

Publication date: December 1, 2003

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