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The water-air interface: a microhabitat for amoebae

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Observations of surface microlayer material collected from freshwaters demonstrate its ability to assemble rapidly at the water-air interface following experimental disruption. This property is exploited to provide a means for studying and manipulating the in situ locomotory behaviour of some surface microlayer amoebae (Acanthamoeba, Naegleria, Vannella) under laboratory conditions. Amoeboid movement is substratum-dependent and so it is important to know the means by which these organisms attach to a water-air interface. Reflection interference microscopy (RIM) demonstrates that during locomotion Vannella adheres to a glass coverslip by means of an unstable platform of associated contact (parallel to, but ∼100-nm off it) within which stable, focal contacts form attaching to the substratum. These two key features of cell-substratum interactions occur also in Acanthamoeba and Naegleria. Direct examination of cell-substratum interactions during amoeboid movement at the water-air interface by RIM proves that the associated contact is also established here.
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Document Type: Research Article

Affiliations: Biology Department, Darwin Building, University College London, Gower Street, London WC1E 6BT, U.K.;, Email: [email protected]

Publication date: 2003-12-01

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