Skip to main content

Changes in the ultrastructure and glycoproteins of the cyst wall of Colpoda cucullus during resting encystment

Buy Article:

$28.45 plus tax (Refund Policy)

Abstract:



Our previous studies on long-term resting encystment of Colpoda inflata showed transcriptional activity of the macronucleus and the occurrence of protein synthesis. In this work we have investigated the structure, the glycoprotein composition, and the lectin-binding site localization of the cyst wall of C. cucullus at increasing cyst ages, by means of scanning and transmission electron microscopy, confocal microscopy, and cytochemical and lectin blotting techniques. During an 18-day resting encystment, the cyst wall undergoes structural modifications resulting in the compaction of its previously-formed layers and the production of new material that is placed on the inner side of the cyst wall. Changes in the localization of ConA-/WGA-binding sites have been checked by comparing 7-day-old cysts to 1-day-old ones. Moreover, differences in the electrophoretic and ConA-/WGA-positive patterns have been found in 18-day-old cysts when compared to 1-day-old ones. The results of in vivo ConA and WGA assays suggest that ConA promotes encystment and delays excystment.

Keywords: Colpoda cucullus; Glycoprotein composition; Lectin-binding sites; Resting cyst wall; Ultrastructural features

Document Type: Original Article

DOI: http://dx.doi.org/10.1078/0932-4739-00888

Affiliations: 1: Department for the Study of the Territory and its Resources, University of Genoa, Corso Europa 26, I-16132 Genoa, Italy; E-mail: gchessa@dipteris.unige.it 2: Department of Ethology, Ecology and Evolution, University of Pisa, Via Volta 4, I-56126 Pisa, Italy 3: Department of Pharmacology & Physiology, Drexel University College of Medicine, NCB 245 N, 15th Street, PA 19102 Philadelphia, USA 4: Department of Experimental, Environmental and Applied Biology, University of Genoa, Viale Benedetto XV 5, I-16132 Genova, Italy

Publication date: December 1, 2002

urban/301/2002/00000038/00000004/art00888
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more