@article {Le:2002:0176-1617:1255,
	author = "Le B.V. and Cruz de Carvalho M.H. and Zuily-Fodil Y. and Thi A.T.P. and Van K.T.T.",
	title = "Direct whole plant regeneration of cowpea [Vigna unguiculata (L.) Walp] from cotyledonary node thin cell layer explants",
	journal = "Journal of Plant Physiology",
	volume = "159",
	number = "11",
	year = "2002",
	abstract = "<P>A simple protocol was established for high frequency direct shoot regeneration of cowpea [<I>Vigna unguiculata</I> (L.) cv. EPACE-1]. Bud proliferation occurred at the cotyledonary nodes of cowpea seedlings three weeks after culture on a medium containing Murashige and Skoog salts (1962) and B5 vitamins (Gamborg et al. 1968) supplemented with TDZ. A 10 <IMG SRC="/iso-ents/isogrk1/mgr-s.gif" ALT="mgr">mol/L TDZ pre-treatment, shoot tip removal and excision of longitudinal thin cell layers (TCL) at the level of the cotyledonary nodes with subsequent culture on a MSB5 medium supplemented with 1 <IMG SRC="/iso-ents/isogrk1/mgr-s.gif" ALT="mgr">mol/L IBA and 1 <IMG SRC="/iso-ents/isogrk1/mgr-s.gif" ALT="mgr">mol/L TDZ were the optimal conditions for maximum bud proliferation. Up to 32.5 regenerated shoot buds were produced per TCL. The regenerated plants (R<SUB>0</SUB>) were true-to-type and successfully transferred to soil.</P>",
	pages = "1255-1258",
	url = "http://www.ingentaconnect.com/content/urban/271/2002/00000159/00000011/art00789",
	doi = "doi:10.1078/0176-1617-00789",
	keyword = "Bud proliferation, cotyledonary nodes, cowpea, thin cell layers"
}