Light and sugar regulation of the barley sucrose : fructan 6-fructosyltransferase promoter

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In this Subject: Botany
By this author: Nagaraj V.J. ;&nbsp; Riedl R. ;&nbsp; Boller T. ;&nbsp; Wiemken A. ;&nbsp; Meyer A.D.

Authors: Nagaraj V.J.; Riedl R.; Boller T.; Wiemken A.; Meyer A.D.

Source: Journal of Plant Physiology, Volume 158, Number 12, December 2001 , pp. 1601-1607(7)

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Abstract:

Fructans are a class of major storage compounds in many plants including several economically important cereals. Earlier we characterized and cloned the sucrose : fructan 6-fructosyltransferase (6-SFT) of barley (Hordeum vulgare L.), a key enzyme for the synthesis of branched fructans (graminans) typical of many cereals and temperate forage grasses. Here, we describe the cloning of the barley 6-SFT promoter region, by PCR-based genome walking procedures. Using a promoter-reporter gene construct (uidA encoding bgr -glucuronidase) and microprojectile bombardment of excised barley leaves, we show that the cloned sequence contains the necessary cis acting elements conferring sucrose and light induction of 6-SFT transcription.