Sucrose synthase and sucrose phosphate synthase, but not acid invertase, are regulated by cold acclimation and deacclimation in cabbage seedlings

Authors: Sasaki H.¹; Ichimura K.²; Imada S.³; Yamaki S.⁴

Source: Journal of Plant Physiology, Volume 158, Number 7, July 2001 , pp. 847-852(6)

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Abstract:

Cabbage seedlings acquire freezing tolerance during exposure to low temperature (acclimation), however, it is lost by returning the plants to moderate temperature (deacclimation). Soluble sugars, except myo-inositol, accumulated during cold acclimation in the leaves of cabbage seedlings. During deacclimation for 5 days, sucrose, glucose and fructose contents decreased rapidly to the same levels as before cold acclimation. To identify the enzymes responsible for changes in sugar concentrations, we investigated changes in activities of sucrose synthase (E.C. 2.4.1.13), sucrose phosphate synthase (E.C. 2.4.1.14) and acid invertase (E.C. 3.2.1.26) during cold acclimation and deacclimation. Activity of sucrose synthase during cold acclimation increased up to 3 times the activity before cold acclimation, but decreased to the level of activity before cold acclimation during deacclimation. The activity of sucrose phosphate synthase also increased during cold acclimation, but decreased to the level of activity before cold acclimation during deacclimation. However, the activity of acid invertase decreased gradually during cold acclimation and did not increase to the level of activity before cold acclimation during deacclimation. These results suggest that SS and SPS, but not acid invertase, are regulated by cold acclimation and deacclimation and play important roles in sugar accumulation and acquisition of freezing tolerance in the leaves of cabbage seedlings.

Keywords: Brassica oleracea L.; freezing tolerance; invertase; sucrose phosphate synthase; sucrose synthase

Language: English

Document Type: Miscellaneous

DOI: http://dx.doi.org/10.1078/0176-1617-00391

Affiliations: 1: Department of Vegetable Breeding, National Research Institute of Vegetables, Ornamental Plants and Tea, Shimokuriyagawa, Morioka, Iwate 020-0123, Japan 2: Department of Floriculture, National Research Institute of Vegetables, Ornamental Plants and Tea, Ano, Mie 514-2392, Japan 3: Department of Applied Physiology, National Research Institute of Vegetables, Ornamental Plants and Tea, Ano, Mie 514-2392, Japan 4: Laboratory of Horticulture, School of Agriculture, Nagoya University, Chikusa, Nagoya, 464-8601, Japan

Publication date: 2001-07-01

Sucrose synthase and sucrose phosphate synthase, but not acid invertase, are regulated by cold acclimation and deacclimation in cabbage seedlings

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