High-level expression of a single-chain Fv fragment (scFv) antibody in transgenic pea seeds

Authors: Saalbach I.; Giersberg M.; Conrad U.

Source: Journal of Plant Physiology, Volume 158, Number 4, April 2001 , pp. 529-533(5)

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Abstract:

The field pea (Pisum sativum L.) is a protein-rich crop and pea seeds are well suited for the production of recombinant proteins. Here we show, that recombinant antibodies can be accumulated in transgenic pea seeds in a homozygous transgenic line up to 2 % of total soluble seed protein. The expression was controlled by the seed-specific USP promoter and the transgenic single-chain Fv antibody protein was retended in the endoplasmic reticulum. The stable inheritance, shown by investigation of the high-level accumulation in the R₃ offspring is another important feature of this new antibody production system. The suitability of transgenic pea seeds as an economically potent production system for recombinant antibodies is clearly demonstrated by our results.

Keywords: field pea; recombinant antibodies; expression system; seed-specific promoter

Language: English

Document Type: Miscellaneous

Affiliations: 1: IPK Gatersleben, Corrensstraße 3, D-06466 Gatersleben

Publication date: 2001-04-01