Neural Wiskott Aldrich Syndrome Protein (N-WASP) and the Arp2/3 complex are recruited to sites of clathrin-mediated endocytosis in cultured fibroblasts
Authors: Merrifield C.J.1; Qualmann B.2; Kessels M.M.2; Almers W.3
Source: European Journal of Cell Biology, Volume 83, Number 1, February 2004 , pp. 13-18(6)
Publisher: Urban & Fischer
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Abstract:
Several findings suggest that actin-mediated motility can play a role in clathrin-mediated endocytosis but it remains unclear whether and when key proteins required for this process are recruited to endocytic sites. Here we investigate this question in live Swiss 3T3 cells using two-colour evanescent field (EF) microscopy. We find that Arp3, a component of the Arp2/3 complex, appears transiently while single clathrin-coated pits internalize. There is also additional recruitment of Neural-Wiskott Aldrich Syndrome Protein (N-WASP), a known activator of the Arp2/3 complex. Both proteins appear at about the same time as actin. We suggest that N-WASP and the Arp2/3 complex trigger actin polymerization during a late step in clathrin-mediated endocytosis, and propel clathrin-coated pits or vesicles from the plasma membrane into the cytoplasm.Document Type: Research article
DOI: 10.1078/0171-9335-00356
Affiliations: 1: Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut, USA 2: Department of Neurochemistry and Molecular Biology, Leibniz Institute for Neurobiology, Magdeburg, Germany 3: Vollum Institute L-474, Oregon Health and Sciences University, Portland, Oregon, USA
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