IngentaConnect alphaB-Crystallin phosphorylated at Ser-59 is localized in centro...

Authors: Inaguma Y.¹; Ito H.¹; Iwamoto I.¹; Saga S.²; Kato K.¹

Source: European Journal of Cell Biology, Volume 80, Number 12, December 2001 , pp. 741-748(8)

Abstract:

We have reported that the three serine residues in alpha B-crystallin are phosphorylated under various stress conditions. We prepared affinity-purified antibodies recognizing each of the phosphorylated serine residues (Ser-19, Ser-45, and Ser-59, respectively) in alpha B-crystallin with peptides (p19S, p45S, or p59S) that contained the corresponding phosphorylated serine residue. Immunocytochemically anti-p45S antibodies stained the cytoplasm of mitotic cells (J. Biol. Chem. 273, 28 346 – 28 354). We have now found that the anti-p59S antibodies recognize centrosomes and midbodies of dividing cells. alpha B-Crystallin was the only protein recognized by the anti-p59S antibodies in Western blot analyses of isolated centrosome fractions. alpha B-Crystallin phosphorylated at Ser-59 was localized at the microtubule organizing centers by means of double staining with anti- beta -tubulin antibody in aster formation analysis and was co-localized with gamma -tubulin in centrosomes. gamma -Tubulin was co-immunoprecipitated with alpha B-crystallin in U373 glioma cell extracts. On the other hand, the location of the phosphorylated alpha B-crystallin deviated from that of alpha -tubulin or gamma -tubulin in the midbody region. Taken together with the evidences that several chaperones are distributed to centrosomes, these results suggest that alpha B-crystallin as a chaperone might be also involved in the quality control of proteins.

Keywords: alpha B-crystallin; centrosomes; midbodies

Language: English

Document Type: Original article

DOI: 10.1078/0171-9335-00203

Affiliations: 1: Department of Biochemistry, Institute for Developmental Research, Aichi Human Service Center, Aichi/Japan 2: Department of Pathology, Aichi Medical University, Aichi/Japan

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B-Crystallin phosphorylated at Ser-59 is localized in centrosomes and midbodies during mitosis