IngentaConnect Plasmodium vivax and Plasmodium chabaudi: Intraerythrocytic traff...

Authors: Bracho C.¹; Dunia I.²; Romano M.¹; Benedetti E-L.²; Perez H.A.¹

Source: European Journal of Cell Biology, Volume 80, Number 2, February 2001 , pp. 164-170(7)

Abstract:

We have used a monoclonal antibody (mAb 7C5B71) raised against the erythrocytic stages of Plasmodium vivax to identify a 148-kDa P. vivax protein antigen (Pv-148) which crossreacts with an antigenically homologous 190-kDa protein of P. chabaudi (Pc-190). During parasite intraerythrocytic development Pv-148 and Pc-190 are exported into the host cell cytosol and become located in the surface membrane of the infected erythrocyte. Immunofluorescence confocal microscopy and immunoelectron microscopy studies showed that both Pv-148 and Pc-190 are released from the parasite and exported to the host cell cytoplasm in association with tubovesicular membrane (TVM) structures. Fluorescent in vivo labelling of P. chabaudi with Bodipy®-ceramide followed by immunofluorescence staining with the mAb supported the association of antigenically homologous Pc-190 with TVM structures. In the presence of brefeldin A (BFA), secretion of antigenically homologous Pc-190 into the host cell cytoplasm was inhibited and the antigen remained in the parasite cytoplasm. BFA also arrested the maturation of the parasite. Taken together these results suggest that Pv-148 and Pc-190 are related parasite proteins that are transported into the host cell through a BFA-sensitive secretory pathway.

Keywords: Plasmodium vivax; Plasmodium chabaudi; brefeldin A; protein traffic; malaria

Language: English

Document Type: Original article

DOI: 10.1078/0171-9335-00137

Affiliations: 1: Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas/Venezuela 2: Institut Jacques Monod, CNRS-Université Paris 7, Paris/France

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$29.89 plus tax

Plasmodium vivax and Plasmodium chabaudi: Intraerythrocytic traffic of antigenically homologous proteins involves a brefeldin A-sensitive secretory pathway