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Laserinduced Fluorescence Spectroscopy for Real-Time Tissue Differentiation

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Background and Objective: Laser-induced fluorescence spectroscopy (LIF) is an important tool for cancer diagnostics. The aim of the study was to examine whether the comparison of laser-induced spectral characteristics, analysed by a specially designed algorithm, allows an identification of bacteria and of human tissue and furthermore even a determination of tissue in respect of dignity.

Study design/Material and Methods: A 337.1 nm nitrogen laser with a 600 m fibre optic was used as light source to induce fluorescence in bacteria and human normal and pathological tissues (ovary, esophagus and kidney). Fluorescence spectra were obtained by means of a spectrograph and a CCD-detector and analysed by a computer-aided programme. A total of 251 measurements was performed (pulse repetition rate: 30 Hz; pulse duration: 0.5 ns). Based on an algorithm, two types of tissue were opposed to eachother, and a test was established for comparison of several samples to a reference.

Results: With a reproducibility of 100% tissues of different origin could be distinguished. Moreover, differentiation of benign and malignant tissues was possible. Test sensitivity lay above 80%.

Conclusions: The results of this pilot study show that this method of laser-induced spectroscopy might be applicable for discrimination of benign and malignant human tissue. Further examinations are needed to verify the in-vivo practicability of this method and to explain the role and presence of endogenous fluorophores.

Document Type: Research Article


Affiliations: 1: Department of Orthodontics, University Hospital Hamburg-Eppendorf, Hamburg, Germany 2: Department of Oral and Cranio-maxillo-facial Surgery, University of Technology Munich, Klinikum rechts der Isar, Munich, Germany

Publication date: May 1, 2004

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