Skip to main content

Free Content Identification of vitellogenic Atlantic blue marlin (Makaira nigricans) from muscle samples using an ELISA for vitellogenin-derived yolk proteins

Download Article:
(PDF 512.2353515625 kb)
The purpose of this study was to develop a direct sandwich enzyme-linked immunosorbent assay (ELISA) for vitellogenin-derived yolk proteins (VDYP) in muscle tissue of pelagic Atlantic blue marlin. gravid ovaries were extracted with physiologic buffer, and purification steps by chromatography were monitored with a cross-reactive mouse monoclonal antibody produced to swordfish (Xiphias gladius Linnaeus, 1758) VDYP. A polyclonal goat antiserum was produced to the enriched VDYP from which specific IgG was purified by affinity chromatography, using immobilized VDYP as the immunoadsorbent. A direct sandwich ELISA was developed using the purified IgG for both capture and detection of the VDYP in standard samples up to a concentration of 1000 ng/ml, and in physiologic buffer-extracted muscle tissue of test samples, using biotin-streptavidin-peroxidase technology. The assay distinguished vitellogenic from non-vitellogenic females and males in extracts of muscle tissue from 27 specimens collected from different areas of the western North Atlantic Ocean during May–September, 2002–2003. The assay for VDYP, and a second for the fish androgen 11-ketotestosterone are in use to indicate gender of electronically-tagged blue marlin from biopsy samples taken just before fish release.

25 References.

No Supplementary Data.
No Data/Media
No Metrics

Document Type: Research Article

Publication date: 2006-03-01

More about this publication?
  • The Bulletin of Marine Science is dedicated to the dissemination of high quality research from the world's oceans. All aspects of marine science are treated by the Bulletin of Marine Science, including papers in marine biology, biological oceanography, fisheries, marine affairs, applied marine physics, marine geology and geophysics, marine and atmospheric chemistry, and meteorology and physical oceanography.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more