Induction of a transferrin-like protein by iron deprivation in the marine alga Dunaliella tertiolecta
The roles of iron in the growth and physiology of the marine alga Dunaliella tertiolecta were investigated. Comparison of the electrophoretic profiles of total proteins from cells grown under iron-replete or iron-deficient conditions revealed that the abundance of a 38-kDa protein was markedly increased in cells deprived of iron. Electron microscopy also revealed that cells subjected to iron limitation exhibited substantial chlorosis and a defective photosynthetic apparatus. In addition, immunoblot analysis showed that iron deprivation increased the abundance of a 150-kDa protein that reacts with antibodies to human transferrin. With the use of the polymerase chain reaction and primers based on the nucleotide sequences of a gene for a transferrin-like protein in Dunaliella salina, a 9.5-kb fragment of D. tertiolecta genomic DNA that encodes a homologous protein was isolated and sequenced. The fragment isolated from D. tertiolecta comprises 30 exons and contains an open reading frame of 3825 bp that encodes a protein of 1274 amino acids. The predicted amino acid sequence of the encoded protein shows a high degree of homology to those of various transferring proteins from other organisms.
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Document Type: Research Article
Publication date: 2000-09-01
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