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Free Content Methodology and Measurement of ATP in Coral

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The amount of adenosine triphosphate (ATP) in living coral is important because this universal energy-supply molecule could become a physiological index for coral conservation. Because coral samples usually contain less than 0.01% living tissue by weight, no ATP measuring method had been previously applied to corals. We examined various methods of ATP extraction and a technique to concentrate ATP by column chromatography was developed. We found that extraction with H2SO4 followed by concentration on charcoal-celite column gave a good result. These data were compared with other morphological and physiological parameters of corals such as weight, surface, polyp number, total protein, total organic carbon and chlorophyll a. The high correlation among these data indicate the feasibility of the method. This method may provide a new approach—a physiological parameter for coral conservation.

Document Type: Research Article

Publication date: September 1, 1987

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  • The Bulletin of Marine Science is dedicated to the dissemination of high quality research from the world's oceans. All aspects of marine science are treated by the Bulletin of Marine Science, including papers in marine biology, biological oceanography, fisheries, marine affairs, applied marine physics, marine geology and geophysics, marine and atmospheric chemistry, and meteorology and physical oceanography.
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