Skip to main content

Free Content Barnacle settlement inhibitors from sea pansies, Renilla reniformis

Download Article:
(PDF 456.8 kb)


Laboratory-reared barnacle larvae Balanus amphitrite were used in settlement assays to isolate and partially purify barnacle settlement inhibitors from extracts of sea pansies, Renilla reniformis. Settlement inhibitors are specific low molecular weight substances. Active substances absorb in the UV and cannot be stained with phosphomolybdate. They have relative mobilities on silica in 9:1 methylene chloride/acetonitrile of 0.3 to 0.55. The region with a mobility of 0.55 has an EC50 of 0.5 mg of original material/ml and is the most potent. Relative mobilities of inhibitory substances on reversed phase thin layer chromatography developed with 3:1 acetonitrile/H2O range from 0.45 to 0.65. Substances with mobilities between 0.45 and 0.55 contain the major inhibitory activity. This active material has an EC50 of <2.5 μ/ml. Settlement inhibitors from R. reniformis are different than settlement inhibitors from whip corals Leptogorgia virgulata (Rittschof et al., 1985) in chromatographic properties. Potency (mg original basis) of inhibitors from sea pansies is 10-fold higher than potencies of inhibitors from whip corals.

Document Type: Research Article

Publication date: September 1, 1986

More about this publication?
  • The Bulletin of Marine Science is dedicated to the dissemination of high quality research from the world's oceans. All aspects of marine science are treated by the Bulletin of Marine Science, including papers in marine biology, biological oceanography, fisheries, marine affairs, applied marine physics, marine geology and geophysics, marine and atmospheric chemistry, and meteorology and physical oceanography.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more