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Free Content Nondestructive Determination of Protein, Total Amino Acids, and Ammonia in Marine Sediments

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Abstract:

A method is described whereby the amounts of ammonia and amino acids in sediments are measured by color reaction with hydrindantin–ninhydrin reagent. Both substances are measured together to give a total value. The ammonia in a separate sample diffuses into boric acid and is then treated with the reagent. The total value less the value for ammonia gives the amount of amino acid. The determination of protein involves its solution in NaOH and reaction with biuret reagent to give a product detectable by ultraviolet light. Sensitivity is 0.02-0.150 ยตm NH3-N or amino-N per ml, and 0.02-0.2 mg protein per ml of final solutions.

Document Type: Research Article

Publication date: June 1, 1970

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  • The Bulletin of Marine Science is dedicated to the dissemination of high quality research from the world's oceans. All aspects of marine science are treated by the Bulletin of Marine Science, including papers in marine biology, biological oceanography, fisheries, marine affairs, applied marine physics, marine geology and geophysics, marine and atmospheric chemistry, and meteorology and physical oceanography.
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