Nondestructive Determination of Protein, Total Amino Acids, and Ammonia in Marine Sediments
Abstract:A method is described whereby the amounts of ammonia and amino acids in sediments are measured by color reaction with hydrindantin–ninhydrin reagent. Both substances are measured together to give a total value. The ammonia in a separate sample diffuses into boric acid and is then treated with the reagent. The total value less the value for ammonia gives the amount of amino acid. The determination of protein involves its solution in NaOH and reaction with biuret reagent to give a product detectable by ultraviolet light. Sensitivity is 0.02-0.150 µm NH3-N or amino-N per ml, and 0.02-0.2 mg protein per ml of final solutions.
Document Type: Research Article
Publication date: June 1, 1970
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