In order to fully extract and utilize high bioactive natural L-DOPA (Levo-dihydroxy phenylalanine), the present study was undertaken to explore the potential applicability of ultrasonic waves to isolate L-DOPA from germinated sprouts, extraction kinetics were also preliminarily studied.
Based on single factor experiments, a Box-Behnken design (BBD) was applied to optimize the processing conditions for the ultrasonic-assisted extraction (UAE) of L-DOPA in germinated fava bean sprouts and evaluate the effects of three independent variables (ultrasonic power, liquid-solid ratio
and extraction time on the yield of L-DOPA. The results showed that ultrasonic power was the most important factor, the maximum yield of L-DOPA (1.47%) was found when 2.0 g germinated sprouts was extracted with 30% (by vol.) ethanol-0.1 M acetic acid solution at ultrasonic power 257 W, liquid-solid
ratio 31 mL/g and extraction time 37.4 min. Under the optimum UAE conditions, L-DOPA recovery of the germinated sprouts was a little higher than that of conventional solvent extraction (CSE) and represented almost 0.58-fold increase when compared with ungerminated sprouts, UAE significantly
shortened the time required for the extraction process. The yields of L-DOPA at different extraction time were simulated by three kinetic models, .i e. the film theory, the unsteady diffusion through plant and the empirical equation of Ponomaryov. The film model gave good simulation to the
experimental data with the highest R2 at 0.9928. The results can provide a reference for natural L-DOPA preparation.
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Keywords:
L-DOPA;
extraction;
germinated fava bean;
kinetics;
optimization
Document Type: Research Article
Publication date:
01 October 2012
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Transations of the Chinese Society of Agricultural Engineering(TCSAE), founded in 1985, is sponsored by the Chinese Chemical Society. TCSAE has been indexed by EI Compendex, CAB Inti, CSA. TCSAE is devoted to reporting the academic developments of Agricultural Engineering mainly in China and some developments from abroad. The primary topics that we consider are the following: comprehensive research, agricultural equipment and mechanization, soil and water engineering, agricultural information and electrical technologies, agricultural bioenvironmental and energy engineering, land consolidation and rehabilitation engineering, agricultural produce processing engineering.
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