GLUTATHIONE REDOX STATUS IN THE HUMAN CELL LINE, A549, FOLLOWING INTRACELLULAR GLUTATHIONE DEPLETION AND EXTRACELLULAR GLUTATHIONE ADDITION

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In this Subject: Ecology ,&nbsp; Toxicology
By this author: Pendergrass J. A. ;&nbsp; Srinivasan J. V. ;&nbsp; Clark E. P. ;&nbsp; Kumar K. S.

Authors: Pendergrass J. A.; Srinivasan J. V.; Clark E. P.; Kumar K. S.

Source: Toxic Substance Mechanisms, Volume 18, Number 1, 1 January 1999 , pp. 11-20(10)

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Abstract:

The redox status of glutathione (L-gamma-glutamyl-L-cysteinylglycine, GSH) plays an im portant role in a number of different cellular reactions including cellular oxidative stress. Using A549 human sm all cell lung carcinoma fibroblasts, we investigated the role of exogenous GSH on the intracellular GSH/glutathione disulfide (oxidized glutathione, GSSG) redox ratio in GSH-depleted cells by treating with L-buthionine-(S,R)-sulfoximine (BSO). GSH levels decreased after BSO treatment. Although BSO is a well-recognized inhibitor of GSH biosynthesis and has no known effect on GSSG reductase, surprisingly, the levels of GSSG also decreased. Incubation of control or GSH-depleted cells with exogenous GSH did not alter intracellular GSH or GSSG levels to any significant extent. Therefore, the ratio of GSH/GSSG also was not altered significantly either in the controls or the BSO-treated cells. It appears that there m ay be cellular hom eostatic mechanisms that would maintain a constant GSH/GSSG ratio, irrespective of the changes in intracellular GSH concentration.

Language: English

Document Type: Research article

Publication date: 1999-01-01