Authors: Eaton, Matt¹; Gursahani, Hemamalini¹; Arieli, Yehuda¹; Pinkerton, Kent²; Schaefer, Saul³

Source: Journal of Toxicology and Environmental Health Part A, Volume 69, Number 15, August 1 2006 , pp. 1497-1510(14)

Publisher: Taylor and Francis Ltd

Abstract:

Chronic exposure to tobacco smoke is known to impair mitochondrial function. However, the effect of acute tobacco smoke exposure (ATSE) in vivo, as might occur in social settings, on mitochondrial function and calcium handling of cardiac cells has not been examined. It was hypothesized that ATSE might adversely modify mitochondrial function as reflected in mitochondrial energetics, membrane potential, and calcium transport. Mitochondria were isolated from the hearts of adult rats either exposed to 6 h of environmental tobacco smoke (∼60 mg/mm 3 tobacco smoke particles) or sham exposure. To model a calcium stress similar to ischemia/reperfusion, mitochondria were exposed to a Ca 2+ bolus with measurement of membrane potential, energetics, Ca 2+ uptake and release, and redox state. ATSE mitochondria were characterized by significantly higher ADP-stimulated ATP production and a more reduced redox state (NADH ratio) under basal conditions without observed changes in resting Ψ m . Exposure of ATSE mitochondria to Ca 2+ stress resulted in significantly more rapid depolarization of Ψ m . The initial rate of Ca 2+ uptake was not altered in ATSE mitochondria, but CsA-sensitive Ca 2+ release was significantly increased. ATSE does not significantly alter resting mitochondrial function. However, ATSE modifies the response of cardiac mitochondria to calcium stress, resulting in a more rapid depolarization and subsequent release of Ca 2+ via the mitochondrial permeability transition (MPT).

Document Type: Research article

DOI: http://dx.doi.org/10.1080/15287390500364788

Affiliations: 1: Division of Cardiovascular Medicine, University of California, Davis, California, USA 2: Center for Health and the Environment, University of California, Davis, California, USA 3: Cardiology Section, Department of Veteran Affairs, Northern California Health Care System, Sacramento, California, USA

Publication date: 2006-08-01

More about this publication?

• Information for Authors
• Subscribe to this Title
• ingentaconnect is not responsible for the content or availability of external websites

Related content

• In this: publication
• By this: publisher
• In this Subject: Ecology ,  Public Health ,  Toxicology
• By this author: Eaton, Matt ;  Gursahani, Hemamalini ;  Arieli, Yehuda ;  Pinkerton, Kent ;  Schaefer, Saul

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers