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Bacterial Diversity in Soil Exposed to Highway Runoff and De-icing Agents

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Abstract:

Bacterial communities were profiled through two drilled soil cores below an infiltration basin that receives highway runoff and de-icing agents. Analyses of groundwater dissolved oxygen, physical and chemical properties of the soil complemented molecular phylogenetic determinations to distinguish ambient and contaminated plume zones. The bacterial community was previously characterized (when the site received high levels of acetate as de-icing agent) by being dominated by members of Geobacteraceae family. In this study, bacterial 16S rDNA gene clones showed highly diverse microbial communities, both into the plume and in the ambient aquifer, in which Geobacter spp. represents only a small fraction of them. The clones were affiliated with 32 and 23 classes identified from the sediment cores along the contaminant plume. The plume in the infiltration basin was anaerobic and iron-reducing, while the sediments in the underlying ambient aquifer were dominated by aerobes due to the presence of aerated ambient groundwater. These data indicate shifts in microbial communities in correlation with depth, substrate and oxygen availability in a de-icing agent impacted subsurface.

Keywords: 16S rDNA gene clone library; bacterial diversity; highway runoff; iron reduction

Document Type: Research Article

DOI: https://doi.org/10.1080/01490451.2010.504608

Affiliations: 1: Civil and Environmental Engineering Department, University of Massachusetts, Amherst, Massachusetts, USA 2: Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA

Publication date: 2011-05-01

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