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Comparison of PROSPECT and HPLC estimates of leaf chlorophyll contents in a grapevine stress study

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This letter describes how PROSPECT, updated with a 1 nm chlorophyll absorbance database, was used to infer total chlorophyll content from the measured reflectance spectra of grapevine leaves. Modelled and measured reflectance spectra agreed on average to within 13% over the range 400–1600 nm. However, discrepancies between modelled and measured reflectances of up to 40% were observed in the spectral region dominated by chlorophyll content. In terms of estimating total chlorophyll content, PROSPECT gave estimates that were on average within 10 µg cm -2 of those obtained using high performance liquid chromatography.

Document Type: Research Article


Affiliations: 1: CSIRO Office of Space Science and Applications, Canberra, ACT 2601, Australia 2: Institut National des Sciences Appliquées de Strasbourg, 24 Boulevard de la Victoire, 67084 Strasbourg Cedex, France

Publication date: February 20, 2006

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