Skip to main content

Open Access A comparison of fermentation in the cyanobacterium Microcystis PCC7806 grown under a light/dark cycle and continuous light

Download Article:
(PDF 278.119140625 kb)
The cyanobacterium Microcystis PCC7806, grown under continuous light, fermented endogenously stored glycogen to equimolar amounts of acetate and ethanol when incubated anaerobically in the dark. In addition, H2, CO2 and some l-lactate were produced. This fermentation pattern differed from that displayed by cells which had been grown under an alternating light/dark (16/8 h) cycle. Such cells produced much more ethanol than acetate, while no lactate was formed. These differences could not be related to the levels of key enzymes of fermentation, which were identical in the two cultures. The cultures grown under continuous light contained twice as much glycogen as the light/dark-grown cells and the former metabolized it at a rate approximately 3 times as fast as the latter culture. Fermentation in the culture grown under continuous light showed low carbon recovery (59-80%) and high oxidation/reduction balance (approximately 1.5). On the basis of calculations of ATP yield it was concluded that this culture was capable of growth driven by fermentation. The increase in structural cell material would account for the missing carbon and reduction equivalents.
No References for this article.
No Supplementary Data.
No Article Media
No Metrics

Keywords: Cyanobacterium; Embden-Meyerhof-Parnas pathway; acetate kinase; alcohol dehydrogenase; dark metabolism; fermentation; hydrogenase; lactate dehydrogenase; light/dark cycle; pyruvate: ferredoxin oxidoreductase

Document Type: Research Article

Affiliations: Netherlands Institute of Ecology, Centre for Estuarine and Coastal Ecology, PO Box 140, 4400 AC Yerseke, The Netherlands

Publication date: 1997-11-26

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more