DNA methylation during antheridial filament development and spermiogenesis in Chara vulgaris (Charophyceae) analysed by in situ nick-translation driven by methylation-sensitive restriction enzymes
DNA of Chara vulgaris thalli contains target sequences for the methylation-sensitive restriction enzymes, Sau3A, HpaII and MspI. These enzymes were used with the in situ nick-translation radioactive technique to evaluate by grain counting in autoradiograms changes in cytosine methylation during antheridial filament development and spermiogenesis. In successive generations of dividing antheridial filaments the level of DNA methylation gradually increases, then decreases slightly in the early stages of spermiogenesis. Spermatozoids display the highest level of DNA methylation.
Keywords: Chara vulgaris; antheridial filaments; in situ nick-translation; methylation-sensitive restriction enzymes
Document Type: Research Article
Publication date: 18 August 1997
- Access Key
- Free content
- Partial Free content
- New content
- Open access content
- Partial Open access content
- Subscribed content
- Partial Subscribed content
- Free trial content