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Twisting DNA: single molecule studies

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Over the past 10 years a number of new techniques have emerged that allow the manipulation of single DNA molecules and other biopolymers (RNA, proteins, etc.). These experiments have permitted the measurement of the DNA stretching and twisting elasticity and have consequently revealed the essential role played by the DNA mechanical properties in its interactions with proteins. We shall first describe the different methods used to stretch and twist single DNA molecules. We will then focus on its behaviour under torsion, especially by discussing the different methods used to estimate its torsional modulus.
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Document Type: Research Article

Affiliations: 1: LPS, ENSUMR 8550 CNRS 24 rue Lhomond 75231 Paris Cedex 05 France 2: Cold Spring Harbor Laboratory 1 Bungtown Road Cold Spring Harbor, NY11724 USA

Publication date: 2004-09-01

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