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Tissue enzyme loci for routine genotyping in Norway pout ( Trisopterus esmarkii Nilsson, 1855): electrophoresis conditions, tissue manifestations, allele designations and basic population genetic parameters

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In order to find polymorphic tissue enzymes and suitable buffer systems for use in population genetic studies of Norway pout ( Trisopterus esmarkii ), a pilot study was performed using 30 individual specimens from one location. A set of potentially useful genetic markers was established, based on electrophoretic analysis of 22 different tissue enzymes in three different buffer systems. The initial analyses revealed 31 putative loci, from which eight were considered to be reliable in routine scoring. Here, a total of 1797 specimens of Norway pout, from 23 individual locations in the fjords and coastal waters of Norway, were scored for these eight loci. Variant alleles were found at all loci. At least five of the loci were polymorphic by the 0.99 criterion ( LDH-2 *, GPI-1 *, GPI-2 *, G3PDH-2 *, IDHP-2 *) and one locus also by the 0.95 criterion ( LDH-2 *). The frequency of polymorphic loci were P 0.99  ≥ 0.625 and P 0.95  = 0.125. The average heterozygosity over all loci based on Hardy-Weinberg expectations was H = 0.072 (±0.050). Here the details of electrophoretic conditions, staining procedures and tissue manifestation of the loci and the allele designations are described.
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Keywords: Gadidae; Norway pout; Trisopterus esmarkii; electrophoresis; heterozygosity; isozymes; population genetics

Document Type: Research Article

Publication date: 01 December 2004

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