An approach to the knowledge of pollen and allergen diversity through lipid transfer protein localisation in taxonomically distant pollen grains
Authors: Suarez-Cervera, MarIa1; Vega-Maray, Ana2; Castells, Teresa1; RodrIguez-Rajo, F. Javier3; Asturias, Juan4; Le Thomas, Annick5; Seoane-Camba, Juan1
Source: Grana, Volume 47, Number 4, December 2008 , pp. 272-284(13)
Publisher: Taylor and Francis Ltd
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Abstract:
The aim of the present study is to localise non-specific lipid transfer proteins (nsLTPs), obtained from Rosaceae (Prunus persica (L.) Batsch) in pollen grains of taxonomically distant plants, such as Iridaceae (Aristea latifolia G.J. Lewis), Platanaceae (Platanus acerifolia (Aiton) Willd.) and Urticaceae (Parietaria judaica L.), in order to compare pollen and nsLTP diversity. A combination of transmission electron microscopy, immunocytochemical techniques, and rabbit specific antiserum against peach nsLTPs (Pru p 3) were used. Abundant labelling to Pru p 3-like proteins was observed in the cytoplasm, walls and pollenkitt of A. latifolia pollen grains. The presence of nsLTPs associated with the pollenkitt proves that it takes part in the defence mechanism of pollen grains. The labelling was less intense in the cytoplasm and walls of P. acerifolia. Immuno-stained gold particles were associated with the vacuoles, lipid inclusions, endoplasmic reticulum and Golgi complex. No significant labelling was found in the P. judaica pollen grains incubated with anti-Pru p 3 polyclonal antibodies. These results indicate important variations in the nsLTPs of different pollen species. Consequently, no taxonomic relationship between pollen grains and nsLTPs could be established.Keywords: Allergens; lipid bodies; lipid transfer proteins; pollen grains; pollenkitt; Pru p 3
Document Type: Research article
DOI: 10.1080/00173130802513776
Affiliations: 1: Department of Botany Faculty of Pharmacy, University of Barcelona, Spain 2: Department of Biodiversity and Environmental Management (Botany), Faculty of Biology, University of Leon, Spain 3: Department of Plant Biology and Soil Sciences, Sciences Faculty of Ourense, University of Vigo, Ourense, Spain 4: R & D Department, Bial-Aristegui S.A., Bilbao, Spain 5: Museum National d'Histoire Naturelle, Paris, France
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