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A novel approach for in situ bud transformation of Populus by Agrobacterium

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Abstract:

Transgenic poplar shoots were obtained using Agrobacterium-mediated in situ bud transformation. The method is demonstrated for the first time for poplar. Buds of Populus cathayana Rehd were co-cultivated with an Agrobacterium strain LBA4404 harbouring the binary vector pBI101-Bmk-chi. With the procedure described here, transformation efficiencies of 1% and 2.24% were achieved in 2006 and 2007, respectively. Stable integration of the transgene sequence was confirmed by polymerase chain reaction and Southern hybridization. The transgenes could be inherited by vegetative propagation. The novel approach circumvented tedious tissue culture procedures and was simple and versatile; hence, it could be readily integrated into conventional tree breeding, especially for species or cultivars not responding to tissue culture.

Keywords: Agrobacterium-mediated; in situ bud transformation; poplar

Document Type: Research Article

DOI: https://doi.org/10.1080/02827580903228870

Affiliations: 1: Biotechnology Institute of Shanxi University, Taiyuan, China,The Agri-Biotechnology Research Centre of Shanxi Province, Taiyuan, China,Shanxi Normal University, Linfen, China 2: Biotechnology Institute of Shanxi University, Taiyuan, China,The Agri-Biotechnology Research Centre of Shanxi Province, Taiyuan, China 3: The Agri-Biotechnology Research Centre of Shanxi Province, Taiyuan, China 4: Biotechnology Institute of Shanxi University, Taiyuan, China

Publication date: 2010-02-01

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