Pinus contorta plants regenerated from adventitious buds induced on zygotic embryos were planted in a field trial where growth, straightness and flowering were followed over 7 years. Seedlings were taller than the plants derived from adventitious buds at the start of the field trial. Adventitious plants did not catch up with seedlings in height after 7 years in the field, but the relative height growth was equal. The higher frequency of plagiotropic growth that was displayed by adventitious plants the first year in the field was not reflected by differences in the frequency of basal sweeps 6 years later. However, the frequency of crooked stems was comparatively high but similar among the plant types. Flowering was more abundant for the seedlings than for adventitious plants. Taken together, the results show that P. contorta can be cloned via adventitious buds from zygotic embryos, and that the resulting plants show satisfactory growth for inclusion in breeding.
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genetic improvement programmes;
Document Type: Research Article
Forestry Research Institute of Sweden, Svalöv, Sweden
Department of Molecular Biosciences, Swedish University of Agricultural Sciences, Uppsala Biomedical Centre, Uppsala, Sweden
Department of Information Technology, Uppsala University, Uppsala, Sweden
Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden
Publication date: 01 August 2005
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