Assessment of potato proteinase inhibitor-II gene as an antifungal and insecticidal agent
The aim of this study was to investigate the protection role of potato proteinase inhibitor-II gene against insect and fungal pathogens of cotton. The positive integration of pinII was confirmed by polymerase chain reaction and Southern analysis in transgenic tobacco plants. In insect bioassay, the transgenic lines were more resistant to cotton bollworm (Helicoverpa armigera) and caused insect mortality and severe growth inhibition. Expression against Rhizoctonia implicated a pivotal role of this gene in defence towards pathogens. Potato proteinase inhibitor-II had shown significant expression against insect as well as fungal pathogens and suggests the role of pinII in engineering insect/pathogen-resistant plants.
Keywords: Bt; Helicoverpa armigera; Rhizoctonia; pinII; tobacco
Document Type: Research Article
Affiliations: 1: National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan 2: Department of Biology, McMaster University, Hamilton, ON, Canada
Publication date: 01 January 2011
- Editorial Board
- Information for Authors
- Subscribe to this Title
- Ingenta Connect is not responsible for the content or availability of external websites
- Access Key
- Free content
- Partial Free content
- New content
- Open access content
- Partial Open access content
- Subscribed content
- Partial Subscribed content
- Free trial content