Phytoestrogen Content of Birdsfoot Trefoil and Red Clover: Effects of Growth Stage and Ensiling Method

Authors: Linda Sarelli1; Mikko Tuori2; Ilkka Saastamoinen1; Liisa Syrjälä-qvist2; Hannu Saloniemi1

Source: Acta Agriculturae Scandinavica, A, Volume 53, Number 1, February 2003 , pp. 58-63(6)

Publisher: Taylor and Francis Ltd

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Abstract:

The phytoestrogen content of birdsfoot trefoil (Lotus corniculatus L.) and red clover (Trifolium pratense L.) was determined. In addition, the effects of growth stage, wilting and ensiling additives on content were studied. Birdsfoot trefoil raw material and silage contained only traces of phytoestrogens. In red clover raw material and silage, the phytoestrogen content varied from 0.8% to 1.1% of dry matter. Content was affected by growth stage of the plant and wilting. Formononetin concentration decreased as the plant matured from budding to flowering stage, and wilting from 25% to 40% of dry matter content decreased genistein and biochanin A content. The phytoestrogen content of red clover silage was 18% higher than that of raw material. Silage additives also affected the content. Silage ensiled with an additive containing Lactobacillus plantarum bacterial inoculate had higher concentrations of genistein and biochanin A than silage made with formic acid.

Keywords: biochanin A; formononetin; genistein; Lotus corniculatus; silage; Trifolium pratense

Document Type: Research article

DOI: http://dx.doi.org/10.1080/09064700310002053

Affiliations: 1: University of Helsinki Faculty of Veterinary Medicine, Department of Clinical Veterinary Science FIN-00014 P.O. Box 57 University of Helsinki 2: University of Helsinki Department of Animal Science FIN-00014 P.O. Box 28 University of Helsinki

Publication date: 2003-02-01

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