Comparison of an enzyme-linked immunospecific assay (ELISA) with the cold complement fixation test for the serodiagnosis of Brucella ovis infection
An enzyme-linked immunospecific assay (ELISA) for the serodiaguosis of Brucella ovis infection in sheep is described and compared with the cold complement fixation (CF) test. ELISA was performed in microtiter plates, using horse-radish peroxidase conjugated to anti-normal sheep serum globulins, and hydrogen peroxide plus o-phenylenediamine as substrate. A heated, cell-free B. ovis extract was used as antigen in both tests. ELISA was easier to perform, distinguished better between positive and negative sera, and did not need heat-inactivated sera.
Keywords: Bacterial; Brucella; Diagnostic procedures; Reproduction - female; Reproduction - male; Serology; Sheep
Document Type: Research Article
Publication date: 01 January 1984
- Subscribe to this Title
- Terms & Conditions
- New Zealand Veterinary Journal on SciQuest
- Ingenta Connect is not responsible for the content or availability of external websites
- Access Key
- Free content
- Partial Free content
- New content
- Open access content
- Partial Open access content
- Subscribed content
- Partial Subscribed content
- Free trial content