Efficient Synthesis of Methanesulphonate-Derived Lipid Chains for Attachment of Proteins to Lipid Membranes

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Abstract:

We have developed an easy and flexible synthetic methodology to obtain lipid chains containing methanothiosulfonate terminal groups with the aim to attach them to natural proteins as functional groups. There are many proteins found in nature that are modified by lipids, and this is a key part of their function. For example, the prion protein is attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor, and this protein is thought to be the causative agent in diseases such as bovine spongiform encephalopathy (BSE; “mad cow disease”) and the human equivalent Creutzfeldt-Jakob disease. However, production of large amounts of protein in bacteria results in proteins that lack these lipid modifications. The lipid chains containing methanothiosulfonate terminal groups that we have synthesized here can be attached to these proteins through the thiol contained in the side chain of the cysteine residue, which can be incorporated into the protein sequence at the desired position.

Keywords: GPI-anchor; Glycosylphosphatidylinositol; prion

Document Type: Research Article

DOI: http://dx.doi.org/10.1080/00397910802213794

Affiliations: 1: Department of Biological Sciences, University of Warwick, Coventry, UK,Department of Chemistry, University of Warwick, Coventry, UK 2: Department of Biological Sciences, University of Warwick, Coventry, UK 3: Department of Inorganic Chemistry, Faculty of Chemistry, University of Santiago de Compostela, Santiago de Compostela, Galicia, Spain

Publication date: January 1, 2008

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