DPPH (1,1-diphenyl-2-picryhydrazyl) radical scavenging assay was used to screen different fractions of Neo-Taraxacum siphonanthum ethanol extracts. The potent active fraction was isolated and purified by preparative high-speed counter-current chromatography (HSCCC) with a solvent system
composed of n-hexane-n-butanol-water (3:4:7, v/v/v). The flow rate was 1.5 mL/min and resolution speed was 800 rpm. Three flavonoid glycosides with the purity over 99% were obtained and identified as luteolin- 3'-O-β-D-glucopyranoside (I), luteolin-7-O-β-D-glucopyranoside
(II), and luteolin-4'-O-β-D-glucopyranoside (III) by ESI-MS, 1H NMR and 13C NMR analysis. Antioxidant activity of three flavonoid glycosides was assessed by DPPH assay, all of which showed potent activity.
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Document Type: Research Article
School of Chemistry and Chemical Engineering, Central South University, Changsha, China
School of Chemistry and Chemical Engineering, Central South University, Changsha, China,Pharmacogenetics Institute of Central South University, Changsha, China
Publication date: 2010-01-01