Enrichment and Purification of Total Chlorogenic Acids from Tobacco Waste Extract with Macroporous Resins

In the present study, an evaluation was conducted on the performance and separation characteristics of nine macroporous resins for the enrichment and purification of total chlorogenic acids from tobacco (Nicotiana tobaccum L.) waste extracts. Based on the results, XAD-4 offered higher adsorption and desorption capacities for total chlorogenic acids than other resins. To optimize the separation process of total chlorogenic acids, a column packed with XAD-4 resin was used to perform dynamic adsorption and desorption tests. The results show that the highest purity of the total chlorogenic acids product was 89.27% when optimum parameters for the adsorption process packed with the XAD-4 resin were as follows: flow rate 3.6 BV/h, pH 3.0; for desorption: ethanol-water (40:60, v/v) used as eluent, flow rate 3.6 BV/h, respectively. Therefore, the XAD-4 resin revealed a good ability to enrich and purify total chlorogenic acids. The method developed will provide a potential approach for the large-scale separation and purification of chlorogenic acid in pharmaceutical applications as a medical intermediate or a material for traditional Chinese medicine.