One Step Purification of Piperine Directly from Piper nigrum L. by High Performance Centrifugal Partition Chromatography

Authors: Chen, Zhe¹; Wu, Jian Bing²; Zhang, Jie¹; Li, Xiao Juan¹; Shen, Min He¹

Source: Separation Science and Technology, Volume 44, Number 8, June 2009 , pp. 1884-1893(10)

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Abstract:

In this study, a preparative high performance centrifugal partition chromatography (HPCPC) method for isolation and purification of the bioactive component piperine directly from the ethanol extract of Piper nigrum L. was successfully established by using n-hexane-ethyl acetate-methanol-water as the two-phase solvent system. The upper phase of n-hexane-ethyl acetate-methanol-water (6:5:6:5, v/v) was used as the stationary phase of CPC. Under the optimum conditions, 40 mg of piperine at 98.5% purity, as determined by HPLC, was yielded from 300 mg of the crude extract in a single CPC separation. The peak fraction of CPC was identified by 1H NMR and 13C NMR.

Keywords: High performance centrifugal partition chromatogra; piperine; Piper nigrum L; preparative chromatography

Document Type: Research article

DOI: http://dx.doi.org/10.1080/01496390902775877

Affiliations: 1: National Clinical Research Base of Traditional Chinese Medicine, Zhejiang Hospital of Traditional Chinese Medicine, Zhejiang Chinese Medical University, Hangzhou, China 2: Institute of Biotechnology, Zhejiang University, Hangzhou, China

Publication date: 2009-06-01