High-Purity Calycosin-7-O--D-glucopyranoside Recovered from Radix Astragali by Extraction, Fractionation and Recrystallization

$61.20 plus tax (Refund Policy)

Buy Article:

Abstract:

Calycosin-7-O--D-glucopyranoside (CG), one major isoflavonoid in Radix Astragali with promising pharmacological effects, was separated with a low-cost process in the present study. The sequential separation and purification procedures established involved extraction with 90% (v/v) aqueous ethanol at 75°C for 2 h twice followed by partition with ethyl acetate, elution with water, and 40% (v/v) aqueous ethanol on a styrene-based resin column and recrystallization at 4°C for 12 h with methanol. These conditions resulted in recovery of 81.6% of total CG with over 97% purity.

Keywords: Calycosin-7-O--D-glucopyranoside; Radix Astragalis; extraction; fractionation; recrystallization; resin

Document Type: Research Article

DOI: http://dx.doi.org/10.1080/01496390902728785

Affiliations: 1: College of Engineering, China Agricultural University, Beijing, P.R. China 2: Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China

Publication date: January 1, 2009

Related content

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more