High-Purity Calycosin-7-O-β-D-glucopyranoside Recovered from Radix Astragali by Extraction, Fractionation and Recrystallization

Authors: Xiao, Wei-hua¹; Han, Lu-jia¹; Shi, Bo²

Source: Separation Science and Technology, Volume 44, Number 5, January 2009 , pp. 1261-1271(11)

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Abstract:

Calycosin-7-O-β-D-glucopyranoside (CG), one major isoflavonoid in Radix Astragali with promising pharmacological effects, was separated with a low-cost process in the present study. The sequential separation and purification procedures established involved extraction with 90% (v/v) aqueous ethanol at 75°C for 2 h twice followed by partition with ethyl acetate, elution with water, and 40% (v/v) aqueous ethanol on a styrene-based resin column and recrystallization at 4°C for 12 h with methanol. These conditions resulted in recovery of 81.6% of total CG with over 97% purity.

Keywords: Calycosin-7-O-β-D-glucopyranoside; extraction; fractionation; Radix Astragalis; recrystallization; resin

Document Type: Research article

DOI: http://dx.doi.org/10.1080/01496390902728785

Affiliations: 1: College of Engineering, China Agricultural University, Beijing, P.R. China 2: Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China

Publication date: 2009-01-01