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High-Purity Calycosin-7-O--D-glucopyranoside Recovered from Radix Astragali by Extraction, Fractionation and Recrystallization

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Calycosin-7-O--D-glucopyranoside (CG), one major isoflavonoid in Radix Astragali with promising pharmacological effects, was separated with a low-cost process in the present study. The sequential separation and purification procedures established involved extraction with 90% (v/v) aqueous ethanol at 75°C for 2 h twice followed by partition with ethyl acetate, elution with water, and 40% (v/v) aqueous ethanol on a styrene-based resin column and recrystallization at 4°C for 12 h with methanol. These conditions resulted in recovery of 81.6% of total CG with over 97% purity.
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Keywords: Calycosin-7-O--D-glucopyranoside; Radix Astragalis; extraction; fractionation; recrystallization; resin

Document Type: Research Article

Affiliations: 1: College of Engineering, China Agricultural University, Beijing, P.R. China 2: Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P.R. China

Publication date: 2009-01-01

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