Chitin Extraction from Black Tiger Shrimp ( Penaeus monodon ) Waste using Organic Acids
Abstract:In chitin extraction from black tiger shrimp shell waste, HCl is the commonly used decalcifying agent. However, it is considered a harsh chemical. An alternate was found by using organic acids. Conditions for deproteinization were: 1 M NaOH at 95°C for 6 h and solid‐to‐solvent ratio of 1:15 (w/v). Demineralization involved treatment with 0.25 M HCl at ambient temperature for 30 min with agitation. The optimal solid‐to‐acid ratio was 1:30 (w/v) and this led to 86.5±1.2% purification of chitin. With the same conditions, the optimal ratio of mixed acids (0.25 M HCOOH and 0.25 M C 6 H 8 O 7 at 1:2 (v/v)) was 1:28 (w/v) with chitin purification of 88.1±1.8%.
Document Type: Research Article
Publication date: 2006-01-01