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Chitin Extraction from Black Tiger Shrimp ( Penaeus monodon ) Waste using Organic Acids

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In chitin extraction from black tiger shrimp shell waste, HCl is the commonly used decalcifying agent. However, it is considered a harsh chemical. An alternate was found by using organic acids. Conditions for deproteinization were: 1 M NaOH at 95°C for 6 h and solid‐to‐solvent ratio of 1:15 (w/v). Demineralization involved treatment with 0.25 M HCl at ambient temperature for 30 min with agitation. The optimal solid‐to‐acid ratio was 1:30 (w/v) and this led to 86.5±1.2% purification of chitin. With the same conditions, the optimal ratio of mixed acids (0.25 M HCOOH and 0.25 M C 6 H 8 O 7 at 1:2 (v/v)) was 1:28 (w/v) with chitin purification of 88.1±1.8%.

Keywords: Chitin; demineralization; depolymerization; extraction; shrimp waste

Document Type: Research Article


Affiliations: 1: School of Engineering, University of Guelph, Guelph, ON, Canada 2: Guelph Food Research Center, Agriculture and Agri‐Food Canada, Guelph, ON, Canada

Publication date: 2006-01-01

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