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HPTLC METHOD FOR QUANTIFICATION OF VALERENIC ACID IN AYURVEDIC DRUG JATAMANSI AND ITS SUBSTITUTES

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Objective of the present study was quantification of valerenic acid in rhizome of three plant species which is generally traded under the name of Jatamansi. A simple, rapid, cost-effective and accurate high performance thin layer chromatographic method has been developed for quantification of valerenic acid in Valeriana jatamansi, Nardostachys jatamansi, and Selinum vaginatum, which is one of the stable compounds and designated as a key marker compound. Separation and quantification of valerenic acid was achieved by HPTLC using ternary mobile phase of toluene: ethyl acetate: formic acid (80:20:5┬áv/v/v) on precoated silica gel 60F254 aluminum plate and densitometric determination was carried out in 280 absorption-reflectance UV mode by deuterium lamp.
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Keywords: HPTLC; Nardostachys jatamansi; Selinum vaginatum; Valeriana jatamansi; valerenic acid

Document Type: Research Article

Affiliations: Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Lucknow, India

Publication date: 01 January 2010

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