HPTLC METHOD FOR QUANTIFICATION OF VALERENIC ACID IN AYURVEDIC DRUG JATAMANSI AND ITS SUBSTITUTES

Authors: Srivastava, Amit; Tiwari, Shashi Shankar; Srivastava, Sharad; Rawat, A. K. S.

Source: Journal of Liquid Chromatography & Related Technologies, Volume 33, Number 18, January 2010 , pp. 1679-1688(10)

Publisher: Taylor and Francis Ltd

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Abstract:

Objective of the present study was quantification of valerenic acid in rhizome of three plant species which is generally traded under the name of Jatamansi. A simple, rapid, cost-effective and accurate high performance thin layer chromatographic method has been developed for quantification of valerenic acid in Valeriana jatamansi, Nardostachys jatamansi, and Selinum vaginatum, which is one of the stable compounds and designated as a key marker compound. Separation and quantification of valerenic acid was achieved by HPTLC using ternary mobile phase of toluene: ethyl acetate: formic acid (80:20:5 v/v/v) on precoated silica gel 60F254 aluminum plate and densitometric determination was carried out in λ280 absorption-reflectance UV mode by deuterium lamp.

Keywords: HPTLC; Nardostachys jatamansi; Selinum vaginatum; valerenic acid; Valeriana jatamansi

Document Type: Research article

DOI: http://dx.doi.org/10.1080/10826076.2010.519250

Affiliations: 1: Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Lucknow, India

Publication date: 2010-01-01