High Performance Liquid Chromatographic Determination of Ginkgotoxin and Ginkgotoxin‐5′‐Glucoside in Ginkgo Biloba Seeds

Authors: Yoshimura, Teruki1; Udaka, Nobuyoshi1; Morita, Junsuke1; Jinyu, Zhang1; Sasaki, Keiko1; Kobayashi, Daisuke1; Wada, Keiji1; Hori, Yasushi2

Source: Journal of Liquid Chromatography & Related Technologies, Volume 29, Number 4, Number 4/2006 , pp. 605-616(12)

Publisher: Taylor and Francis Ltd

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Abstract:

A method for simultaneous determination of ginkgotoxin (4'-O-methylpyridoxin), ginkgotoxin-5'-glucoside, and vitamin B 6 compounds in Ginkgo biloba seeds, has been developed by using high performance liquid chromatography (HPLC) with fluorescent detection. Satisfactory separation was accomplished on a C 18 reversed-phase column by gradient elution with potassium phosphate containing sodium 1-pentanesulfonate (pH 2.5)-acetonitrile within 20 min. The linearity of the calibration curves for ginkgotoxin and ginkgotoxin-5'-glucoside ranged from 0.1 pmol to 10 pmol per injection, and the detection limit was 0.025 pmol (SN=5). Hot water extracts of raw, canned, and plastic packaged Ginkgo biloba seeds obtained in Japan were analyzed by using this method.

Keywords: Ginkgotoxin; Ginkgotoxin‐5′‐glucoside; Ginkgo biloba seeds; HPLC; Fluorescence detection

Document Type: Research article

DOI: 10.1080/10826070500531466

Affiliations: 1: Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Hokkaido, Japan 2: Department of Hospital Pharmacy, Niigata City General Hospital, Niigata, Japan

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