Stability of Opioid Growth Factor ([Met 5 ]‐Enkephalin) in Solution Using HPLC and Photodiode Array Detection

Authors: Jeffrey Hurst, W.¹; McLaughlin, Patricia²; Zagon, Ian²; Rogosnitzky, Moshe³

Source: Journal of Liquid Chromatography & Related Technologies, Volume 29, Number 2, Number 2/2006 , pp. 151-157(7)

Publisher: Taylor and Francis Ltd

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Abstract:

A reversed phased HPLC method with photodiode array detection was used to monitor the stability of opioid growth factor (OGF; [Met 5 ]-enkephalin) in sterile saline for 1, 2, and 8 weeks, and stored at -17°C, 4°C, and 23°C. Samples maintained at -17°C had 97% of the initial OGF concentration after 2 months, whereas those stored at 4°C had 94% of the original concentration. Preparations housed at 22°C had reductions of 6–12% by the 8th week. These data provide important information about storage of OGF for clinical applications.

Keywords: Degradation; HPLC; Stability; Opioid growth factor; Methionine enkephalin

Document Type: Research article

DOI: http://dx.doi.org/10.1080/10826070500416395

Affiliations: 1: Department of Comparative Medicine, Penn State University College of Medicine, Hershey, Pennsylvania 2: Department of Neural and Behavioral Sciences, Penn State University College of Medicine, Hershey, Pennsylvania 3: Telz Stone, Israel

Publication date: 2006-01-01